Architecture of the fungal nuclear pore inner ring complex

Architecture of the nuclear pore complex coat

Molecular Architecture of the Major Membrane Ring Component of the Nuclear Pore Complex.

The membrane ring that equatorially circumscribes the nuclear pore complex (NPC) in the perinuclear lumen of the nuclear envelope is composed largely of Pom152 in yeast and its ortholog Nup210 (or Gp210) in vertebrates. Here, we have used a combination of negative-stain electron microscopy, nuclear magnetic resonance, and small-angle X-ray scattering methods to determine an integrative structur...

The Yeast Nuclear Pore Complex: Composition, Architecture, and Transport Mechanism

An understanding of how the nuclear pore complex (NPC) mediates nucleocytoplasmic exchange requires a comprehensive inventory of the molecular components of the NPC and a knowledge of how each component contributes to the overall structure of this large molecular translocation machine. Therefore, we have taken a comprehensive approach to classify all components of the yeast NPC (nucleoporins). ...

The nuclear pore complex: from molecular architecture to functional dynamics.

Toward dissecting the molecular composition and architecture of the nuclear pore complex (NPC), over the past 18 months novel nucleoporins and NPC subcomplexes were identified and characterized. The three-dimensional structure of isolated yeast NPCs was determined by electron cryomicroscopy. New specimen preparation and labeling protocols localized a number of nucleoporins and NPC subcomplexes ...

The nuclear pore complex

M OLECULAR trafficking between the nucleus and the cytoplasm of interphase eukaryotic cells occurs via the nuclear pore complexes (NPCs), 1 organelles with a roughly cylindrical shape (~,120 um in diameter and ~70-um high) that are embedded in the double-membraned nuclear envelope (NE). The NPC allows passive diffusion of ions and small molecules through an aqueous channel with a physical diame...